Publication of our Flow cytometry systems

Research paper

Takeshi Sawada, Masaru Watanabe, Yuu Fujimura, Shigehiro Yagishita, Tatsu Shimoyama, Yoshiharu Maeda, Shintaro Kanda, Mayu Yunokawa, Kenji Tamura, Tomohide Tamura, Hironobu Minami, Yasuhiro Koh and Fumiaki Koizumi: ;
Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells
Cancer Sci March 2016,vol.107, no. 3, 314


Masaru Watanabe, Masakuni Serizawa, Takeshi Sawada, KazuoTakeda, Toshiaki Takahashi, Nobuyuki Yamamoto, Fumiaki Koizumi and Yasuhiro Koh;
A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood
Journal of Translational Medicine 12(1):143 · May 2014


Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Takeshi Sawada, Kazuo Takeda, Fumiaki Koizumi, Yasuhiro Koh;
Multicolor Detection of Rare Tumor Cells in Blood Using a Novel Flow Cytometry-Based System
Cytometry Part A 85A: 206213, 2014


Wlodkowic D, Skommer J, Akagi J, Fujimura Y, Takeda K;
Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip Flow Cytometry
Curr Protoc Cytom. 2013 Oct 9;66:Unit 9.42


Donald Wlodkowic, Jin Akagi, Jurek Dobrucki, Rachel Errington, Paul J Smith,
Kazuo Takeda and Zbigniew Darzynkiewicz;

Kinetic viability assays using DRAQ7 probe
Curr Protoc Cytom. 2013 July ; 0 9


Jin Akagi, Magdalena Kordon, Hong Zhao, Anna Matuszek, Jurek Dobrucki, Rachel Errington, Paul J Smith,Kazuo Takeda, Zbigniew Darzynkiewicz, and Donald Wlodkowic;
Real-time cell viability assays using a new anthracycline derivative DRAQ7®
Cytometry A. 2013 February ; 83(2): 227–234.


Joanna Skommer, Jin Akagi, KazuoTakeda, Yuu Fujimura, Khashayar Khoshmanesh, Donald Wlodkowic;
Multiparameter Lab-on-a-Chip flow cytometry of the cell cycle.
Biosensors & bioelectronics 11/2012; 42C:586-591


Jin Akagi, Kazuo Takeda, Yuu Fujimura, Anna Matuszek, Khashayar Khoshmanesh, Donald Wlodkowic;
Microflow Cytometry in Studies of Programmed Tumour Cell Death
Procedia Engineering 02/2012; 47:88–91.


Susumu Yoshie, Jun Ito, Sakiko Shirasawa, Tadayuki Yokoyama, Yuu Fujimura, Kazuo Takeda, Masahiro Mizuguchi, Ken Matsumoto, Daihachiro Tomotsune, and Katsunori Sasaki;
Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.
Tissue Engineering Part C Methods 09/2011; 18(1):12-20


Fumie Jimma, Yuu Fujimura and Kazuo Takeda ;
Small-scale cell analysis by the microfluidic flow cytometer, FISHMAN-R, using a disposable chip.
Cytometry Research. 03/2011; 21(1):31-36.


Kazuo Takeda, Yu Fujimura and Fumie Jimma;
Development of a new compact cytometer using a disposable microfluidic chip for contamination-free and biosafety measurement
Cytometry Research. 03/2011; 21(1):43-50.


Masashi Takao and Kazuo Takeda;
Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry;
Cytometry A. 79(2),93-4, 2011


Que Chi Truong-Bolduc, Gilles R. Bolduc, Ryo Okumura, Brian Celino, Jennifer Bevis, Chun-Hsing Liao, and David C. Hooper;
Implication of the NorB Efflux Pump in the Adaptation of Staphylococcus aureus to Growth at Acid pH and in Resistance to Moxifloxacin
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, July 2011, p. 3214–3219


Ken Nishimura, Masayuki Sano, Manami Ohtaka, Birei Furuta, Yoko Umemura, Yoshiro Nakajima, Yuzuru Ikehara, Toshihiro Kobayashi, Hiroaki Segawa, Satoko Takayasu, Hideyuki Sato, Kaori Motomura, Eriko Uchida, Toshie Kanayasu-Toyoda, Makoto Asashima, Hiromitsu Nakauchi, Teruhide Yamaguchi and Mahito Nakanishi;
Development of Defective and Persistent Sendai Virus Vector: a Unique Gene Delivery/Expression System Ideal for Cell Reprogramming;
J. Biol. Chem. 2011, 286:4760-4771.


Kazuo Takeda and Fumie Jimma;
Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Cytometry B. 76B 405-406. 2009


Conference presentation & poster

Y. Fujimura, T. Ino, T. Tanaka, J. Akagi, Y. Bansho, R.Watanabe, M. Ishige and K. Takeda;
Image Recognition Cell Dispensing System for Plating of Single Cells and Monodisperse Spheroids
CYTO2016, B274, Seattle, Washington, USA, June 11–15, 2016

Y. Bansho, J. Akagi, T. Ino, K. Osawa, T. Tanaka, M.Ishige, Y. Fujimura and K. Takeda;
The Emulsion Sorting Technology Platform for High-Throughput Screening Assays in Various Fields of Biology
CYTO2016, B138, Seattle, Washington, USA, June 11–15, 2016

J. Akagi, Y. Fujimura, Y. Kawase and K.Takeda;
Water-in-Oil Emulsion Sorting for Digital PCR and Single Cell Analysis Using Air Pressure Pulse Flow in Disposable Microfluidic Chip
CYTO2015, B235, Glasgow, Scotland, June 26–30, 2015

T. Ino, H. Zhang, M. Ishige, K. Aiba, Y. Fujimura, N. Nakatsuji and K. Takeda;
Negative Cell Sorting for Removal of Undifferentiated Cells for Regenerative Medicine
CYTO2015, B242, Glasgow, Scotland, June 26–30, 2015

Yasuhiro Koh, Masaru Watanabe, Takeshi Sawada, Hirotsugu Kenmotsu, Masakuni Serizawa, Masayuki Ishige, Kazuo Takeda, Nobuyuki Yamamoto, Toshiaki Takahashi and Fumiaki Koizumi;
A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of circulating tumor cells
AACR Annual Meeting 2014; April 5-9, 2014; San Diego

Takeshi Sawada, Yukiko Ito, Masaru Watanabe, Yuu Fujimura, Shinji Nakamichi, Shintaro Kanda, Hidehito Horinouchi, Yutaka Fujiwara, Hiroshi Nokihara, Noboru Yamamoto, Tomohide Tamura, Yasuhiro Koh, Fumiaki Koizumi;
Clinical feasibility study of a novel cytometry-based-system for the detection of circulating tumor cells (CTCs) of patients with lung cancer
AACR2013 #1445 Washington, DC, April 6-10, 2013


Masaru Watanabe, Takeshi Sawada, Masakuni Serizawa, Yukiko Ito, Yuu Fujimura, Fumiaki Koizumi, Yasuhiro Koh;
Molecular characterization of captured circulating tumor cells (CTCs) with a novel cell sorting system
AACR2013, #5105 Washington, DC, April 6-10, 2013


Yukiko Ito, Takeshi Sawada, Masaru Watanabe, Yuu Fujimura, Jun Hashimoto, Makoto Kodaira, Mayu Yunokawa, Harukaze Yamamoto, Kan Yonamori, Chikako Shimizu, Kenji Tamura, Yasuhiro Fujiwara, Yasuhiro Koh, Fumiaki Koizumi;
Detection of circulating tumor cells for malignant epithelial tumor using a novel cytometry-based system
AACR2013, # 1446 Washington, DC, April 6-10, 2013


Jin Akagi, Joanna Skommer, Anna Matuszek, Kazuo Takeda, Yuu Fujimura, Khashayar Khoshmanesh, Kourosh Kalantar-Zadeh, Arnan Mitchell, Rachel Errington,Paul J Smith, Zbigniew Darzynkiewicz, and Donald Wlodkowic;
Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry
SPIE Microfluidics, BioMEMS, and Medical Microsystems XI, San Francisco, California, USA, February 2, 2013


Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Junko Suzuki, Fumiaki Koizumi, Yasuhiro Koh;
Evaluation of a novel flow cytometry-based system for the detection of circulating tumor cells (CTCs)
AACR2012, #1735 Chicago, March31-April 4, 2012


Yuu Fujimura and Kazuo Takeda;
Development of disposable microfluidic chip based cell sorter for high cell purification with no damage
CYTO2011,B144, Baltimore, USA, May 21-25, 2011


Kazuo Takeda and Namiko Yamashita;
Cell clump analysis for flow cytometric enumeration of circular tumor cells by using disposable microfluidic chip
CYTO2011,B143, Baltimore, USA, May 21-25, 2011


Kazuo Takeda and Fumie Jimma;
Development of cross-contamination free flow cytometer using a disposable multi flow channel chip with side scattered light detection optics.
CYTO2010, P227, Seattle, USA, May 8-12, 2010


Masashi Takao, Fumie Jimma, and Kazuo Takeda;
Enumeration and characterization of circulating tumor cells with cross-contamination free flow cytometer
CYTO2010, P112, Seattle, USA, May 8-12, 2010


Q.C. TRUONG-BOLDUC, G.R. BOLDUC, B. CELINO, J. BEVIS, Y. DING, R.VILLET, and D.C. HOOPER;
Expression of the Staphylococcus aureus Efflux Pumps NorA, B, and C in Response to Acidification
ICAAC2010, September 12-15, in Boston, USA


Kazuo Takeda and Fumie Jimma;
Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Clinical Cytometry Meeting 2009, No31, Jacksonville Florida, October 16 – 21