Reagent

Reagent to prevent cell blockages in the micro flow channels

Directions 1: Sheath liquid adjustment
Sheath liquid is a liquid for flow cytometer with the role to which the focus of the flow of cells are on a line. When analysis/separation by On-chip Flow / On-chip Sort, using the optimal buffer for the cell, you dilute this Reagent with the buffer and use as sheath liquid.

Directions 2 : Prevention from cell adsorption
It prevents a cell’s sticking to a chip or a tube and losing. In various cell experiments, by adding this agent to a buffer, coating a chip and a tube beforehand, the cell adsorption will be prevented.

Through Path Plus (reagent to prevent cell blockages in the micro flow channels)
(10mL x6/box)

cell sorter reagent
Product No.: 2001004-6
Product Name:  Through Path Plus 40× (10mL x6)
Purpose: During measurement and sorting in On-chip Flow/Sort, dilute this product with culture medium or buffer to use it as sheath fluid.
Add this product in advance to the culture medium or buffer in use, to prevent cell adhesion. Coating a chip or tube with this product prevents
cells from adhering.
Usage: Use fluid diluted 40-fold with culture medium or buffer (PBS etc.) as sheath fluid and sample buffer
Storage conditions: Store at 4°C (store refrigerated for not more than one month after opening)

Price: Please contact us
Note We have confirmed that this product is non-toxic to most cells in normal conditions

Sheath fluid

It is sheath liquid of Ready-to-use. “Through pass plus” of the prevention from cell adsorption is included beforehand. Moreover, since non-serum cell preservation liquid is used, it is sheath liquid with few damages to the cell.
(Sheath liquid is a liquid for flow cytometer with the role to which the focus of the flow of cells are on a line.)

On-chip Standard Sheath Fluid (50mLx4/box)

Onchip sheath
Product No.: 2001008
Product Name: On-chip Sheath (50mL)
Purpose:

Sheath fluid consisting of Through Path Plus 40x diluted 40-fold with proprietary serum-free cell preservation fluid.
Preparation is not required. Cells sorted with this product can be stored in a refrigerator for a few days.
During measurement and sorting in On-chip Flow/Sort use this as sheath fluid.
Also, coating a chip or tube with this product prevents cells from adhering.

Usage: Use this as a sheath fluid and sample buffer.
This is a mixture close to a culture solution, so open it in a clean bench.
Storage conditions: Store at 4C
(store refrigerated for not more than one month after opening)

Price: Please contact us

Sample Buffer

Sample buffer is buffer included PVP in order to suppress sedimentation of a cell.
It is used for cell analysis and cell separation using by On-chip Flow / On-chip Sort
There are two kinds of sample buffer.
One is “On-chip Standard Sample Buffer “, it is Ready-to-use, included “Through pass plus” of the prevention from cell adsorption and non-serum cell preservation liquid beforehand.
The other is “Sample buffer 2x “ , it is used by two fold dilution with the target buffer.

On-chip On-chip Standard Sample Buffer (10mLx4/box)

cell sorter buffer
Product No.: 2001009
Product Name: On-chip sample buffer(10mL)
Purpose: A solution in which Through Path Plus 40x and PVP (final concentration 1.5%) are diluted with proprietary serum-free cell preservation fluid
Preparation is not required.
Sorted cells can be stored in a refrigerator for a few days.
Usage: Put the cells in suspension to produce a sample fluid.
*This is a mixture close to a culture solution, so open it in a clean bench
Storage conditions: Store at 4  C
(store refrigerated for not more than one month after opening)
Price: Please contact us
Note1 The PVP concentration must be accurately 1.5%, so rinse the cells two or three times with On-chip Sample buffer to replace them. PVP makes it difficult to centrifuge cells, so it is necessary to centrifuge at 1.5–3 times the normal speed.

Note2 We have confirmed that this product is non-toxic to most cells in normal conditions

Sample buffer 2x (50 mL)

cell sorter Buffer
Product No.: 2001005
Product Name:  On-chip sample buffer 2x (50mL)
Purpose: A solution for sample buffer preparation, containing 3% PVP.
Wash the cells two or three times in a fluid diluted twofold with the intended medium, to replace and suspend them.
Through Path Plus is not included, so it may be added if the application calls for it.

Usage: Wash the cells two or three times in a fluid diluted twofold with the intended medium, to replace and suspend them.
Storage conditions: Store at 4 C
(store refrigerated for not more than one month after opening)
Price: Please contact us
Note1: The final PVP concentration must be accurately 1.5%.

Note2 We have confirmed that this product is non-toxic to most cells in normal conditions